Shan Ju Lin was dumped after posting on Facebook that “gays should be treated as patients” and “abnormal sex behaviour leads to abnormal crime”.
In another post, Ms Lin derided outgoing US President Barack Obama’s LGBTI policies using a digitally manipulated picture of him in drag.
One Nation party officials wrote to Ms Lin on Saturday night via Facebook Messenger to inform her of the party’s decision.
“The office has had no ability to contact you, so I’m unfortunately left with the task of letting you know party officials are not happy with your comments,” the official wrote to Ms Lin.
“The party executive and review committee will be following this matter up and determining your endorsement for the Queensland state election.”
In a statement party leader Pauline Hanson said Lin has failed to follow the party’s request that she stop posting disparaging content relating to LGBT people.
“These are not the views shared by One Nation, nor the views of fellow candidates and the general public,” Senator Hanson said in a statement.
“I will not stand by and allow people to trash the party or my name, so I make no apologies for being tough on candidates.”
Lin responded saying: “Once gays realise they can put pressure on a candidate, they will start to target other One Nation candidates also.”
Shan Ju Lin told her Facebook followers that she would not compromise her values and would continue advocate for the views they shared.
Ms Lin is the second candidate to fall out with One Nation less than a month after it unveiled part of the team it would take to the 2018 Queensland election.
She said her “wholehearted support” for Ms Hanson remained.
“I look forward to the internal issues within One Nation being finally resolved for good,” she said.